早期梅毒血清IFN-γ、IL-18和IL-13水平检测

摘要

Objective: To assess the role of IFN - γ, IL- 18 and IL- 13 in the cellular immunity of early syphilis. Method: The serum levels of IFN - γ, IL- 18 and IL- 13 were measured by DAbS - ELISA in 50 patients with early syphilis and 52 healthy individuals. Results: The serum levels of IFN - γ, IL - 18 and IL - 13 were higher in early syphilis than in control group ( P ＜ 0.05), serum level of IFN - γ and IL- 18 was higher in primary syphilis than in secondary syphilis, early latent syphilis and control group (P ＜ 0.05). The serum level of IL- 13 was higher in secondary syphilis than in primary syphilis and control group ( P ＜ 0.01), but lower than in early latent syphihs ( P ＜ 0.05); The titer of RPR test was not correlated with the serum levels of IFN - γ, IL- 18 and IL - 13 ( P ＞ 0.05); The correlation was negative between IFN - γ and IL - 13 ( P ＜ 0.05), positive between IFN -y and IL- 18 (P ＜0.01). There was no correlation between IL- 18 and IL- 13 ( P＞ 0.05). Conclusion: The changes of IFN - γ, IL- 18 and IL - 13 may be involved in the imbalance of Thl/Th2 immune responses in early syphilis.%目的:评价IFN-γ、IL-18和IL-13在早期梅毒细胞免疫中的作用.方法:采用DAbS-ELISA法检测50例早期梅毒患者和52例健康人血清IFN-γ、IL-18和 IL-13水平.结果:早期梅毒组IFN-γ、IL-18和IL-13水平高于对照组(P<0.05);一期梅毒组IFN-γ、IL-18水平高于二期、早期潜伏梅毒组和对照组(均P<0.05),二期梅毒组IL-13水平高于一期梅毒组和对照组(P<0.01),低于早期潜伏梅毒组(P<0.05);RPR试验滴度与IFN-γ、IL-18和IL-13不相关(P>0.05);IFN-γ和IL-13负相关(P<0.05),IFN-γ和IL-18正相关(P<0.01),IL-18和IL-13不相关(P>0.05).结论:早期梅毒血清IFN-γ、IL-18和IL-13的变化可能是导致早期梅毒发生Th1/Th2免疫应答失衡的重要原因.