左西替利嗪对外周血衍生的树突状细胞表达CD86和分泌IL-8的影响

摘要

Objective:To detect the effects of levocetirizine on the expression of CD86 and production of IL-8 by monocyte-derived dendritic cells ( MoDC) . Methods: Monocytes were isolated from healthy volunteers and were induced into monocyte-derived dendritic cells (MoDC). MoDC was cultured with histamine and levocetirizine separately. The secretion of CD86 and IL-8 by MoDC in the co-cultures was detected by FACS and ELSIA methods, separately. Results: In the culture with histamine, the secretion of CD86 cells and IL-8 was increased,while in the culture with levocetirizine the secretion of CD86 cells and IL-8 was reduced (P<0.05). However, this suppressive effect varied in different subjects. Conclusions: Levocetirizine can suppress the increase of CD86 cells and IL-8 secretion after histamine stimulation. The clinical treatment with levocetirizine should be individually.%目的：确定左西替利嗪对组胺诱导的单核细胞衍生的树突状细胞( MoDC)表达CD86和分泌IL-8的影响。方法：分离健康志愿者外周血单核细胞,进行体外诱导培养获得MoDC,以组胺作进一步诱导,并加入左西替利嗪进行干预,以FACS和ELSIA检测MoDC中CD86表达和IL-8分泌。结果：经组胺诱导后,MoDC的CD86表达水平和 IL-8分泌量明显增多,左西替利嗪干预后CD86表达和IL-8分泌均受到不同程度的抑制( P<0.05)。此外,不同个体来源的MoDC对药物的敏感性也存在差异。结论：左西替利嗪的抗组胺作用机制是抑制组胺诱导的MoDC表达CD86和分泌IL-8,但临床药物治疗应个体化。