目的：：明确CXCL12对人黑素瘤细胞株M14体外增殖、迁移、侵袭的影响。方法：用不同浓度的CXCL12(0、10、50、100、200 ng/mL)刺激或诱导人黑素瘤细胞株M14，CCK8法检测细胞增殖， Transwell迁移和侵袭试验检测细胞迁移和侵袭能力。用100 ng/mL的CXCL12处理人黑素瘤细胞株M14，Western blot法检测处理后不同时间点(0、5、10、20、30、60 min)的 ERK、p－ERK、AKT、p－AKT的表达。结果： CXCL12能够增强人黑素瘤细胞株M14细胞的体外增殖、迁移、侵袭能力，以及促进ERK、AKT信号通路的激活。结论： CXCL12可能与黑素瘤的进展过程相关。%Objective:To investigate the effects of CXCL12 on the biological behavior of human melanoma M14 cell line. Methods:The human melanoma M14 cell line was incubated or induced by different concentra￣tions of CXCL12 ( 0,10,50,100,200 ng/mL) . The Cell Counting Kit-8 ( CCK8) , transwell migration and in￣vasion assay were used to evaluate the proliferation, migration, and invasion of M14 cells. The Western blot was used to detect the protein expression of ERK, p-ERK, AKT, and p-AKT of M14 cells treated with 100 ng/mL CXCL12 at different times ( 0, 5, 10, 20, 30, 60 min) . Results: CXCL12 promoted the prolifera￣tion, migration and invasion of M14 cells, and the activation of the ERK and AKT signaling pathways. Conclusion:CXCL12 may be associated with the progression of melanoma.
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