[Objective] To study the influence of laparoscopic surgery to cervical cancer cell proliferation capacity.[Methods] Using flow cytometry technology and real-time fluorescence quantitative RT-PCR,the cervical cancer cell apoptosis rate and the change of N-myc,Fas gene expression levels were detected in 20 cervical cancer patients before and after laparoscopy (laparoscopic group).Their specimens were compared with those from 20 cervical cancer patients who received open surgery (open surgery group) and the normal cervix from 20 cases who underwent laparoscopic hysterectomy(laparoscopic hysterectomy group).[Results] ①In the laparoscopic group,the preoperative and postoperative cervical cancer cell apoptosis rates were (4.50±0.88)％ and (31.37±1.66)％ respectively with significant difference (P＜0.001); in the open surgery group the preoperative and postoperative cervical cancer cell apoptosis rates were (4.38±0.67)％ and (4.31±0.53)％ respectively with no significant difference (P＞0.05); in the laparoscopic hysterectomy group,the preoperative and postoperative apoptosis rates of normal cervical cells were (16.95±1.64)％ and (17.12±1.57)％ respectively without significant difference (P ＞0.05).②In the laparoscopic group the N-myc,Fas gene expressions of preoperative cervical cancer tissue were (0.014±0.010) and (0.007±0.003) respectively,the postoperative gene expressions were (0.002±0.002) and (0.022±0.011) respectively,the differences were significant (P＜0.001); in the open surgery group N-myc,Fas gene expressions in preoperative cervical cancer tissue were (0.006±0.003) and (0.008±0.003) respectively,the postoperative gene expressions were (0.006±0.002)and (0.008 ±0.003) respectively,there were no significant differences (P ＞0.05); in the laparoscopic hysterectomy group N-myc,Fas gene expressions in preoperative normal cervical tissue were (0.001±0.000) and (0.024±0.007) respectively,the postoperative gene expressions were (0.001±0.000) and (0.024 ±0.007) respectively,there were no significant differences (P ＞0.05).[Conclusions] Laparoscopic surgery does not increase but inhibits the proliferation ability of the cervical cancer cells,therefore,it is a safe operation method.%目的 探讨腹腔镜手术对子宫颈癌细胞增殖能力的影响.方法 分别采用流式细胞技术及实时荧光定量RT-PCR检测20例子宫颈癌患者腹腔镜术前、术后子宫颈癌细胞的凋亡率及N-myc、Fas基因表达水平的改变,并与20例子宫颈癌患者开腹手术组及20例腹腔镜下全子宫切除术组正常子宫颈组织相对照,分别对以上各组标本进行对比分析.结果 ①腹腔镜手术组子宫颈癌细胞术前及术后的凋亡率分别为(4.50±0.88)％和(31.37±1.66)％,差异有显著性(P＜0.001)；开腹手术组子宫颈癌细胞术前及术后的凋亡率分别为(4.38±0.67)％和(4.31±0.53)％,差异无显著性(P＞0.05)；腹腔镜下全子宫切除术组正常子宫颈细胞术前及术后的凋亡率分别为(16.95±1.64)％和(17.12±1.57)％,差异无显著性(P＞0.05).②腹腔镜手术组术前子宫颈癌组织中N-myc、Fas基因的表达量分别为(0.014±0.010)和(0.007±0.003),术后基因的表达量分别(0.002±0.002)和(0.022±0.011),差异有显著性(P＜0.001);开腹手术组术前子宫颈癌组织中N-myc、Fas基因的表达量分别为(0.006±0.003)和(0.008±0.003),术后基因的表达量分别(0.006±0.002)和(0.008±0.003),差异无显著性(P＞0.05)；腹腔镜下全子宫切除术组术前正常子宫颈组织中N-myc、Fas基因的表达量分别为(0.001±0.000)和(0.024±0.007),术后基因的表达量分别(0.001±0.000)和(0.024±0.007),差异无显著性(P＞0.05).结论 腹腔镜手术并不增加子宫颈癌细胞的增殖能力,反而对子宫颈癌细胞的增殖能力有抑制作用,是一种安全的手术方法.
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