绵羊Hoxc8与d11基因甲基化的量子力学特征与功能

摘要

The Hoxc 8 and Hoxd11 genes among Dorset, German Merino, Australia Merino and Mongolia sheep, and the methylation position and density of exon-1 CpGs were determined.For Hoxc8 and Hoxd11 exons in German Merino, Dorset, Australia Merino, there were no different from that of Mongolia sheep, but several differences in the intron for these breeds.The exon-1 sequence of the function genes was related to the quantum mechanics of the molecular orbit(MO) of DNA methylation.The positions and the numbers of methylated cytosine in the second codes and anti-codes of the genes decided the effects of the DNA methylation on the transcription and translation.The methylation rate from rams of 14 thoracic vertebrae Mongolia sheep were higher,and the ewes were lower,but the methylation rate from the parents of 13 thoracic vertebrae Mongolia sheep were both lower.%文章研究了蒙古羊和德国美利奴肉羊、陶赛特肉羊、澳洲美利奴细毛羊的Hoxc8和Hoxd11基因及其第1位外显子的甲基化位置、数量、稳定程度,以及甲基化的量子力学性质.与蒙古羊相比,内含子序列有数个碱基的差异,外显子一致.外显子序列的甲基化在转录和翻译过程中的功能,与甲基化导致的分子轨道和量子力学变化有关.甲基化胞嘧啶在密码与反密码中的第2位,决定甲基化是否对转录和翻译产生效应.蒙古羊14枚胸椎个体的父本Hoxc8 exon-1和Hoxd11 exon-1序列甲基化程度高,母本的甲基化程度低;13枚胸椎个体的父本和母本相应序列的甲基化程度都低.