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芡实壳中脂肪酸及其微量元素的测定

         

摘要

The contents of trace elements and fatty acids in Euryale Ferox shell were analysed. The fatty acids in the Euryale shell were extracted with petroleum ether in Soxhlets extractor, using 0. 5mol/L NaOH-CH3OH as the methyl esterification solvent and separated by GC-MS. The constituents of fatty acids were identified and their amount were determined by normalization method. Sample of Euryale shell was digested with a mixture of HN03 and HC1O4 , and contents of trace elements were determined by ICP-AES. The result shows that 8 compounds were identified, accounting for 83. 72% of its total content. The main components were palmitic acid, linoleic acid and oleic acid, their contents were 10. 72% , 28. 35% and 12. 62%. Fourteen kinds of trace elements were obtained, Euryale shell was rich in Cu, Mn, Zn and Fe with the contents of 0. 248 mg/g, 0. 164 mg/g, 0. 217 mg/g and 0. 305 mg/g.%分析了芡实壳中微量元素和脂肪酸的含量.用石油醚作溶剂在索氏提取器中提取芡实壳的脂肪酸,0.5mol/L氢氧化钠-甲醇溶液做甲酯化处理,气相色谱-质谱联用法进行定性分析,按峰面积归一化法,求出脂肪酸成分的百分含量.经硝酸-高氯酸体系消解,应用电感耦合等离子体原子发射光谱法测定芡实壳微量元素的含量.结果表明,从脂肪酸中鉴定出8个化学组分,和占总成分的83.72%,主要成分为棕榈酸、亚油酸和油酸,含量分别为10.72%、28.35%、12.62%;检测出14种微量元素,其中铜、锰、锌和铁的含量较高,分别为0.248 mg/g、0.164 mg/g、0.217 mg/g和0.305 mg/g.

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