利用SPR技术测定湖水中微囊藻毒素

摘要

Amount of Microcystin-LR in water was rapidly and quantitatively detected by SPR (surface plasmon resonance) technique. After the MC-LR being immobilized on the surface of the chip, the stability of the chip was tested, and the standard curve was constructed by the principle of competitive immune inhibition. The results showed that the relative standard deviation of 50 cycles was 0.62%, which represented the stability of the immobilized chip. And the limited concentration of detection is arrived at 0.25ng/mL For its stability and sensitivity, the SPR detection method would be applied to assay of MC-LR in the water.%利用表面等离子共振(SPR)技术,建立了快速定量检测湖水中微囊藻毒素LR(MC-LR)的方法.将MC-LR共价偶联到表面等离子共振芯片表面,检测了芯片的稳定性,并利用免疫竞争抑制原理构建标准曲线,对湖水样品进行了检测.结果表明:制备的芯片信号稳定,50个循环相对标准偏差(RSD)为0.62％；该方法的最低检测浓度为0.25ng/mL,可作为水体中MC-LR的分析检测方法.