建立了有机相丹酰化衍生-UPLC-MS-MS人体尿液样品中双酚A(BPA)及4种氯代双酚A(氯代BPA)的检测方法,对北京地区40个女性尿液样品进行了检测.结果表明:BPA 和一氯、二氯、三氯、四氯 BPA 的检出率分别为 90%、96%、90%、52%和 45%;浓度分别为(1.30±1.24),(0.40±0.37),(0.41±0.51),(0.18±1.49),(0.46±0.35) ng/mL,4种氯代BPA的总浓度为1.45ng/mL,和BPA浓度相仿.4种氯代BPA的BPA等当量浓度为4.84ng/mL,是BPA浓度的2.2倍. 40个尿液样本BPA等当量浓度符合对数正态分布,超过BPA的糖尿病发生阈值(0.05%发病风险)5.7ng/mL的概率为19.2%.人体暴露氯代BPA的健康风险应该引起重视.%A derivatization UPLC-MS-MS method was used to analyze bisphenol A (BPA) and its chlorinated derivatives, including monochloro-BPA, dichloro-BPA, tricholro-BPA, and tetrachloro-BPA, in 40urine samples of Beijing women. The detection frequencies of BPA, monochloro-BPA, dichloro-BPA, tricholro-BPA, and tetrachloro-BPA were 90%, 96%, 90%, 52%, and 45% with average concentrations of (1.30±1.24), (0.40±0.37), (0.41±0.51), (0.18±1.49), (0.46±0.35) ng/mL, respectively. While the total concentration of four chlorinated BPA was 1.45ng/mL, which was similar with that of BPA in urine, their BPA toxic equivalent quantity (TEQBaP) in urine was calculated to be 4.84ng/mL, which was 2.2 fold higher than that of BPA. The TEQBPA concentration was log-normally distributed, and the probability higher than Benchmark Dose (BMDL0.05), corresponding to the increasing risk of diabetes by 0.05% due to exposure to BPA, was 19.2%. Our results indicated that chlorinated BPA might pose a relatively high risk.
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