季节性温度对短程硝化系统微生物群落的影响

摘要

为了研究季节性温度对短程硝化生物膜系统中微生物群落结构的影响,以平均水温分别为28.3,23.3,21.6,9.6,-0.2℃C条件下MBBR反应器的短程硝化生物膜为对象,利用聚合酶链式反应-变性梯度凝胶电泳技术(PCR-DGGE)对比分析上述生物膜中的微生物群落结构,结果表明:在控制合适的水力停留时间(HRT)条件下,水温降低会影响微生物活性,但不影响短程硝化稳定性,平均水温在21.6～0.2℃变化过程中总细菌和AOB的多样性及均匀性基本不变,芽孢杆菌、黄杆菌、亚硝化单胞菌等污水处理功能微生物都得到保留.平均水温28.3～-0.2℃C条件下生物膜中的优势菌群主要分布于变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)其中AOB均属于β-Proteobacteria的亚硝化单胞菌属(Nitrosomonas).本试验仅调控反应器水力停留时间(HRT)就可以实现低温短程硝化,对于短程硝化工艺广泛应用于工程实践具有重要意义.%In order to investigate the effect of seasonal temperature on microbial community structure of nitrification biofilm system,the nitrification biofilm system in MBBR reactor under the temperature of 28.3,23.3,21.6,9.6 and-0.2 ℃ were studied by using denatures gradient gel electrophoresis polymerase chain reaction(PCR-DGGE) technique.The activity of bacteria affected by loss of the water temperature,but did not affect stability of nitrification.During the change of water temperature(21.6～0.2℃C),the total bacteria and AOB stayed stable;and the waste-water treatment of Bacillus,Flavobacterium and Nitrosomonas also stayed stable.The dominant bacteria in biofilm mainly was Proteobacteria and Bacteroidetes under the average temperature of 28.3～-0.2℃C,and the AOB was Nitrosomonas that was the part of β-Proteobacteria.The partial nitrification under low temperature was achieved by controlling HRT merely,and it made great sense for the application of shortcut nitrification process.