荷斯坦牛Leptin基因外显子2多态性与繁殖力性状的相关性研究

摘要

以94头荷斯坦牛为试验材料,应用PCR-SSCP技术研究Leptin基因外显子2多态性与繁殖力的相关性.在Leptin基因外显子2的E2FB区上,经分析存在影响奶牛繁殖的多态位点,测序首次发现102nt处T突变为C.在平均产犊间隔上,AA与AT差异极显著(P＜0.01);AT与TT差异极显著(P＜0.01);A为优势等位基因,可作为荷斯坦牛育种中繁殖力的分子辅助标记.%PCR-SSCP technology were employed to analyze the correlation between Leptin gene's polymorphism and fertility traits in 94 Holstein cows. The polymorphism(T/C)in the Locus 102bp affecting the cows' fertility was firstly detected in the exon 2 area (E2FB)of Leptin gene,after Sequencing. For the average parity interval,there were significant differences between genotype AA and genotype AT(P＜0.01 ), between genotype AT and genotype TT (P＜0.01). Genotype A was dominant allele, which could he applied for the molecular genetic markers in fertility of Holstein cows.