响应面法优化大蒜多糖的提取工艺研究

摘要

Obj ective:To optimize the extraction technique conditions for polysaccharides from AlliumsativumL..Methods:The singer factor test and response surface methodology (RSM)are applied. The technological parameters are determined according to regression analysis by SAS.Results:The optimum conditions are as follows:extraction temperature of 90 ℃,extraction duration of 4.24 h, and water to material ratio of 1∶35.The optimum extracting rate for polysaccharides is 4.04%.Con-clusion:RSM is feasible for optimizing the extraction conditions of polysaccharides from AlliumsativumL.,which has provided theoretical basis for improving the extraction rate of polysaccharides.%目的：探索大蒜多糖水浸提的最佳提取工艺条件。方法：在单因子实验的基础上，运用合理的实验设计方案，采用响应面法（RSM）优化大蒜多糖的提取条件。结果：依据回归分析确定多糖提取率的影响因子，求得最佳水浸提条件：提取温度为90℃，浸提时间为4．24 h，料液比为1∶35，大蒜多糖的提取率为4．04％。结论：响应面法对大蒜多糖的提取条件优化合理可行，为提高多糖的提取率提供了理论依据。