菌体蛋白水解液应用于谷氨酸发酵的研究

摘要

Glutamic acid bacterial protein is from the temperature-sensitive glutamic acid fermentation residue,after an appropriate amount of soybean meal hydrolyzate hydrolysis substitute for temperature-sensitive glutamic acid fermentation medium.By fermentation process optimization,the experimental results show that adding a certain amount of glutamic acid bacterial protein hydrolyzate in temperature-sensitive glutaric acid fermentation medium,the fermentation acid production and conversion rate have significantly improved.Through process optimization,the temperature-sensitive glutamic acid fermentation medium composition is determined as follows:starch hydrolysed sugar is 55 g/L,corn syrup is 20 mL/L,glutamic acid bacterial protein hydrolyzate is 10 g/L,soybean hydrolyzate is 10 g/L,molasses is 15 g/L,Na2 HPO4 is 7 g/L,KCl is 4 g/L,MgSO4 · 7H2O is 1.5 g/L,MnSO4 · 7H2O is 30 mg/L,FeSO4 · 7H2O is 30 mg/L,vitamin B1 is 350 μg/L,vitamin H is 500 μg/L,antifoaming agent is 0.1 mL/L.Under such culture conditions,the glutamic acid production rate reaches 18 g/L,the conversion rate is 65 ％,and the glutamic acid fermentation temperature-sensitive comprehensive level is increased.%谷氨酸菌体蛋白来自温敏型谷氨酸发酵生产废渣,经水解后替代适量豆粕水解液用于温敏型谷氨酸发酵培养基中.通过进行发酵工艺优化,实验结果表明在温敏型谷氨酸发酵培养基中添加一定量的谷氨酸菌体蛋白水解液,发酵的产酸和转化率有显著的提高.经过工艺优化,温敏型谷氨酸发酵培养基组成确定为:淀粉水解糖55 g/L,玉米浆20 mL/L,谷氨酸菌体蛋白水解液10 g/L,豆粕水解液10 g/L,糖蜜15 g/L,Na2HPO4 7 g/L,KC1 4 g/L,MgSO4·7H2O 1.5 g/L,MnSO4·7H2O 30 mg/L,FeSO4·7H2O 30 mg/L,维生素B1350 μg/L,维生素H 500 μg/L,消泡剂0.1 mL/L.在此培养条件下,谷氨酸的产酸率达18 g/L,转化率达65％,温敏型谷氨酸发酵综合水平有所提高.