气相色谱法检测牛奶中脂肪酸组分方法的改进

摘要

[Objective]The purpose of this study is to find and establish a simple and efficient method used to detect the fatty acid composition and content in the milk. [Method]Gas chromatography （GC-FID） method was used to separate the milk fatty acid composition by using the Agilent HP-88 fatty acid methyl esters capillary column and temperature programmed method, quantitative retention time was used for quantify by qualitative and peak area percentage method. [Result]The results showed that the fatty acid in milk was well separated. The recovery and precision of detection method were 99. 588% and 2. 600, respectively, with a high accuracy and repeatability. [Result]The experimental method could be used as a milk fatty acid detection technology for promotion and application.%[目的]为了找到并建立一套简单而高效的方法用来测定牛奶中脂肪酸的组分和含量。[方法]应用气相色谱（GC-FID）方法,采用Agilent HP-88专用脂肪酸甲酯毛细管柱和程序升温方法来分离牛奶中的脂肪酸组分,用保留时间定性和峰面积百分比法进行定量。[结果]表明：牛奶中的脂肪酸得到了很好的分离,该检测方法的回收率为99.588%,精密度为2.600,具有较高的准确性和重复度。[结论]本实验方法可以作为牛奶中脂肪酸检测的技术加以推广和应用。