以红色糖多孢菌(Saccharopolyspora erythraea)高产突变株N5为研究对象,通过单因素及正交试验结果确定发酵培养基最佳组成为:葡萄糖3％,淀粉3％,玉米浆0.15％,硫酸铵0.7％,豆油0.3mL/25mL.此外,培养条件优化结果为:发酵的最佳接种量10％；最佳装液量50mL/250mL;最佳初始pH值为6.5；最佳培养温度33℃;发酵时间156h.%The optimal fermentation conditions of mutant Saccharopolyspora erythraea N5 producing erythromycin were investigated through single factor and orthogonal experiment. The optimal medium compositions were as followed: glucose 3%, starch 3%, corn plasma 0.15%, ammonium sul-fate 0.7% and soybean oil 0.3ml/5ml. In addition, the optimal erythromycin fermentation condition were as followed: inoculum 10%, medium volume of shaking flask 50mL/250ml, initial pH value 6.5, the culture temperature 33℃ and fermentation time 156h.
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