The effects of continuous fermentation, batch fermentation and fed-batch fermentation on mycelium yield, intracellular and extracellular polysaccharide yields of Antrodia camphorata were studied by submerged fermentation. The results indicated that in comparison to continuous culture, the mycelium yield increased 5.51%, the yield of intracellular polysaccharide increased 11.25%, and the yield of extracellular polysaccharide increased 21.70% by fed-batch fermentation. Besides, the fed-batch fermentation was superior to continuous and batch fermentation.%通过深层发酵法,对比研究了连续、分批、补料分批3种培养方式对樟芝真菌菌体,胞内外多糖产量的影响,研究表明,补料分批相对于连续培养,菌体最大产量提高5.51％,胞内多糖最大产量提高11.25％.胞外多糖最大产量提高21.70％,补料分批培养优于连续培养和分批培养.
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