高致病性猪繁殖与呼吸综合征病毒BJSD株和BJPG株序列分析

摘要

本研究旨在预测与分析高致病性猪繁殖与呼吸综合征病毒BJSD株和BJPG株序列特点,为其下一步研究作准备.利用分子生物学软件及服务器,对BJSD株和BJPG株全序列及各结构蛋白的生化特性、溶解性和核定位信号等进行了分析,结果显示BJSD株和BJPG株至少有12个非结构蛋白和6个结构蛋白；同源性比较结果显示GP3和GP5蛋白变异性高,M和N蛋白保守.GP3、GP5和M蛋白糖基化位点与参考株相比有变化,GP2、GP4和N蛋白糖基化位点与所有参考株一致.服务器预测BJSD株和BJPG株GP2、M和N蛋白生化特性显示各项数据完全一致,其中N蛋白的碱性氨基酸比例明显高于其他蛋白,总平均亲水值明显低于其他蛋白；GP2、GP3和GP4蛋白不稳定,GP5、M和N蛋白稳定.BJSD株和BJPG株可溶性只有GP3和GP5略有差别,其余蛋白数据一致,6个结构蛋白预测不溶性概率均在66％以上,GP3不溶性概率最高.BJSD株和BJPG株结构蛋白核定位结果显示,两株仅GP3和GP5有差异,其余蛋白分布情况完全一致.可以利用以上一些不同于以往各代表株的特点,对PRRS的诊断和防制做进一步的研究.%Predicting and analysising the properties of HP-PRRSV BJSD and BJPG sequence,which will prepare for further study of this strain. Molecular biology software and server are used to analysis the complete sequence of HP-PRRSV BJSD and BJPG strain, the biochemistry properties, solubility of various structural proteins and nuclear location singal (NLS). The results show that there are 12 non-structural and 6 structual protein at least in HP-PRRSV BJSD and BJPG. When we compare different protein homology,we found that GP3 and GP5 are high mutant,M and N are conservative. The protein glycosylation site of GP3, GP5 and M are different from the reference strains, but GP2, GP4 and N are the same. Server shows that biochemical characteristics of GP2 ,M and N protein of BJSD and BJPG strains is identical, the ratio of alkaline amino acid of N is higher than GP2 and M,and the total average hydrophilic value obviously lower than other proteins. GP2,GP3 and GP4 are unstable; GP5, M and N are opposite. The solubility of all proteins of BJSD and BJPG strains is consistent but GP3 and GP5 has somewhat different. Prediction of 6 structural protein insoluble probability are accounted for more than 66 percent,GP3 is highest. The nuclear localization of structural proteins of BJSD and BJPG strains show that distribution of all proteins is identical but GP3 and GP5. The properties of above strains can pave the way for the diagnosis and treatment of PRRS.