高剂量高致病性猪繁殖与呼吸综合征病毒TJM-F92疫苗株对低剂量伪狂犬病毒Bartha-K61疫苗株的免疫干扰研究

摘要

To determine the immune interference between the combination vaccine of high dose of highly pathogenic porcine reproductive and respiratory syndrome virus(HP-PRRSV) TJM-F92 strain and pseudorabies virus(PRV) Bartha-K61 strain against the low dose of PRV Bartha-K61 strain,several parameters including the levels of the humoral immunity,challenge protection and pathological changes have been evaluated.The results demonstrated that the antibody level in the high dose of combination vaccine group was consistent with the low dose of PRV vaccine group.The immune interference between the two groups has not been observed.The challenge protection rate was 100％ (5/5) in both of the combination vaccine group and the PRV group.However,the protection rate was 0％ (0/5) in the control group after the PRV JL1 strain challenge.Only mild lesions have been observed in the tissues of the two vaccinated groups after the challenge.The conclusion is no immune interference between the combination vaccine and PRV vaccine during the vaccination.%为研究高剂量高致病性猪繁殖与呼吸综合征病毒(PRRSV) TJM-F92疫苗株对低剂量伪狂犬病毒(PRV) Bartha-K61疫苗株是否存在免疫干扰作用,本研究对其从体液免疫水平、免疫攻毒保护水平及病理学变化等方面进行评价.结果表明,二联免疫组抗体消长规律与低剂量PRV Bartha-K61株免疫对照组抗体消长规律相一致,二联免疫组中高剂量PRRSV TJM-F92株未抑制低剂量PRV Bartha-K61株抗体生成;二联免疫组与低剂量PRV Bartha-K61免疫对照组对PRV JL1株强毒攻击保护率均为5/5;免疫病理学研究结果表明,两组免疫组攻击PRV JL1株强毒后各组织器官病理变化轻微;二联免疫组与低剂量PRV Bartha-K61免疫对照组均可对PRV强毒攻击产生良好的保护效果.综上,高剂量PRRSV TJM-F92株对低剂量PRV Bartha-K61株无免疫干扰作用.