华南地区两株副猪嗜血杆菌的生物学特性与致病性研究

摘要

Two strains of Haemophilus parasuisv/ere studied on the characterization of colony culture, biological characteristics, serotype identification, resistance to drug, antigenicity and pathogenicity. The serological identification proved that one is 4 antigen type, and the other is 5 antigen type. The results showed that the two strains of Haemophilus parasuis multiplied rapidly and could reach 109 CFU in 10 h. Their LD50 are 1. 52 × 109 CFU and 9. 3 × 108 CFU. These two isolated strains had drug resistance to Fluoroquinolone antimicrobials and Sulfamethoxazole and Trimethoprium (SXT). They all have high immu-nogenicity. These results indicate that these two strains of Haemophilus parasuis could be the candidate vaccine strains.%本试验旨在对来源于广东省的两株副猪嗜血杆菌的生化特性、血清型、耐药性、生长特性以及毒力和免疫原性进行研究.试验结果表明,两株菌分别为血清4型和5型；两株菌均具有较好的生长能力,培养10h活菌含量都能达到109以上；其LD50分别是1.52×109 CFU和9.3×l08 CFU;分离菌株对喹诺酮类和磺胺类抗生素有较强的抵抗力；免疫试验结果表明,两株菌具有较好的免疫原性.以上试验结果表明,这两株菌可以作为疫苗的基础候选菌株.