野生与家养乌苏里貉FSHβ和FSHR基因的克隆及序列分析

摘要

In wild and domestic Wusuli raccoon dog, the FSHβ gene partial sequence, FSHR gene 5' upstream regulatory region and exon 10 were amplified and sequenced.The length of these sequences were 1257, 757 and 1398 bp, respectively, the GenBank accession numbers were HQ385977, HQ385978 and HQ385979.The homology were 99％, 98％ and 98％ for the exon sequences of these three genes with dog by BLAST.By multiple alignment analysis of these three genes with DNAMAN software, 12, 10 and 3 variable sites were detected, there were 2, 1 and 0 mutation sites occurred in the coding region.6, 9 and 2 polymorphic sites of these three genes were detected comparing two wild raccoon dogs, respectively.While only 2, 1 and 0 polymorphic sites occurred for two domestic raccoon dogs.The results demonstrated that wild raccoon showed more genetic diversity.%对野生和家养乌苏里貉的FSHβ基因部分序列、FSHR基因5′端上游调控区和外显子10进行扩增和测序,获得序列长度分别为1257、757和1398 bp,提交GenBank,登录号分别为HQ385977、HQ385978和HQ385979.BLAST比较分析3段序列外显子区,与犬的同源性分别为99%、98%和98%.利用DNAMAN软件对2只野生与2只家养貉进行多序列比对,结果分析,3个基因片段中分别检测到12、10和3个变异位点,其中编码区碱基的突变位点分别有2、1和0个;将2只野生貉的序列相比较,发现3段序列分别有6、9和3个多态位点,而将2只家养貉的序列相比后分别有2、1和0个多态位点,说明野生貉更具有遗传多样性.