石房蛤毒素单克隆抗体的制备及其特性研究

摘要

将石房蛤毒素(Saxitoxin,STX)与牛血清白蛋白(BSA)偶联构建完全抗原STX-BSA,免疫BALB/c小鼠,通过杂交瘤技术获得1株稳定分泌抗STX单克隆抗体的杂交瘤细胞株,单抗亚类为Igm.体内诱生法收集腹水单抗,间接ELISA方法测定抗体效价为1:102400,抗体亲和常数为3.71×10(6) L/mol.%Saxitoxin(STX)was conjugated to bovine serum albumin (BSA) as immune antigen.Inoculated mouse with STXBSA, gained one hybridoma cell strains secreting monoclonal antibodies against STX stably.The subclass of the McAb was IgM.The titer of the McAb was 1∶ 102400 in ascites according to the indirect ELISA determination.The affinity constant was 3.71 × 106 L/mol.