试验选用30头“军牧1号”猪,随机分成5组,包括常温对照组和4个冷应激组.常温组在21℃±2℃饲养,冷应激组的温度设置分别为:-10℃±2℃、-5℃±2℃、0℃±2℃、5℃±2℃.冷应激2h屠宰后采集腹腔脂肪、颈部皮下脂肪、胸部皮下脂肪和肝脏组织样品,通过荧光定量PCR方法检测细胞因子信号转导抑制因子3(suppressor of cytokine signaling 3,SOCS3)的mRNA的表达量,初步探讨冷应激对猪脂肪及肝脏组织中SOCS3 mRNA表达量的影响.试验结果显示,冷应激猪SOCS3在腹腔脂肪、颈部皮下脂肪、胸部皮下脂肪和肝脏组织中,随着温度的降低,表达量逐渐升高,且总体差异显著(P＜0.05).试验结果表明,SOCS3受到冷应激的影响,在不同脂肪组织部位发生了变化,可能参加了脂肪细胞因子的调控,从而改变脂肪组织分布及脂肪代谢平衡,为研究冷应激对机体的影响及作用机制奠定了试验依据和理论基础.%The samples of the adipose layer in the neck and bosom, adipose of abdominal cavity, liver of pigs were obtained at 2 h after the temperature of -10 ℃±2 ℃ , -5 ℃±2 ℃ , 0 ℃±2 ℃ , 5 ℃±2℃ , 21 ℃±2 ℃ (the control) to measure suppressor of cytokine signaling 3(SOCS3) gene expression by the method of the Real-time PCR in order to evaluate the effect of cold stress on expression of SOCS3 in liver and adipose. The results showed that mRNA of the adipose layer in the neck and bosom, adipose of abdominal cavity, liver of pigs were of significant difference in all the groups(P<0. 05). It was concluded that SOCS3 was sensitive to cold stress which might regulate the balance of adipose and the transduction of signalling pathway related with fat metablisom. This study revealed the role of regulation of SOCS3 on the adipose metabolism of pigs during cold stress, and provided the experimental foundation and theoretical support to study the effect of cold stress and mechanism of this signaling pathway on animal.
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