罗格列酮对哮喘小鼠气道炎症和肺组织细胞因子信号抑制因子3和5mRNA表达的影响

摘要

Aim:To investigate the effects of rosiglitazone on asthma in mice and elucidated the potential relationship between the effects and expression of suppressor of cytokine signaling(SOCS) 3 and SOCS5 mRNA. Methocds: Asthma model was established by OVA sensitization in mice. A totle of 24 mice were randomly divided into three groups :saline control group( group A) ,asthmatic group( group B) and rosiglitazone group( group C) ,with 8 mice in each group. Pathologic changes of lung were examined by HE staining. IL-4 and IFN-y contents were examined by enzyme-linked immunosorbent assay.SOCS3 and SOCS5 mRNA expression were detected by reverse transcription-polymerase chain reaction. Restults:There were many inflammatory cells infiltration in bronchial walls and lumen as well as around blood vessels and bronchus in group B.Compared with that in group A,The concentration of IL-4 in group B was significantly higher, the concentration of IFN-γ was significantly decreased ( P ＜ 0.05 ). Compared with that in groups B, the concentration of IL-4 was significantly lower and the concentration of IFN-γ was significantly higher in group C ( P ＜ 0.05 ). Compared with that in group A, SOCS3 mRNA expression were significantly higher and the SOCS5 mRNA expression were significantly lower in group B ( P ＜0.05). Compared with that in group B, the SOCS3 mRNA expression was significantly reduced and the SOCS5 mRNA expression was significantly increased in group C ( P ＜ O. 05 ). Conclusion: Rosiglitazone could relieve the allergic inflammation of airway. Effects of rosiglitazone could involve inhibiting SOCS3 mRNA expression and upgrading SOCS5 mRNA expression.%目的:研究罗格列酮对哮喘小鼠气道炎症的影响.方法:24只BALB/c小鼠分为正常对照组(A组)、哮喘模型组(B组)和罗格列酮组(C组),每组8只.B和C组通过卵清蛋白(OVA)致敏建立小鼠哮喘模型,C组建模同时吸入罗格列酮5×10<'-5>mol/L.HE染色观察肺组织病理学形态;ELISA方法检测小鼠肺泡灌洗液(BALF)中自细胞介素4(IL-4)和干扰素-γ(IFN-γ)的含量;RT-PCR方法检测小鼠肺组织细胞因子信号抑制因子(SOCS)3和SOCS5 mRNA的表达.结果:B组小鼠气道黏膜充血水肿,黏膜层增厚,支气管壁及血管壁周围有较多炎细胞浸润;C组仅有少量炎细胞浸润.3组IL-4和IFN-γ含量比较,差异有统计学意义(F=112.234和58.977,P均<0.001),与A组比较,B组IL-4含量增多,IFN-γ含量减少(P均<0.05);与B组比较,C组IL-4含量减少,IFN-γ含量增多(P均<0.05).3组SOCS3和SOCS5 mRNA 含量比较,差异有统计学意义(F=38.746和87.235,P均<0.001),与A比较,B组SOCS3 mRNA 的表达增高,SOCS5 mRNA 的表达降低(P均<0.05);与B组比较,C组SOCS3 mRNA的表达减少,SOCS5 mRNA表达的增加(P均<0.05).结论:罗格列酮能抑制哮喘小鼠气道炎症反应,其作用机制可能与抑制SOCS3和增加SOCS5的mRNA表达有关.