首页> 中文期刊>中国畜牧兽医 >猪大肠杆菌和葡萄球菌的分离鉴定及耐药性分析

猪大肠杆菌和葡萄球菌的分离鉴定及耐药性分析

     

摘要

对湘西北地区某猪场患病的仔猪的粪便及病变的脾脏、肝脏、淋巴结、肺脏、肾水肿液进行病原菌分离鉴定,并用Kirby-Bauer法测定分离菌株对15种抗菌药的敏感性.结果表明,患病仔猪分离到大肠杆菌和猪葡萄球菌两种细菌.其中大肠杆菌对庆大霉素、头孢呋肟、丁胺卡那霉素、四环素、先锋必素敏感,对氯霉素、氧哌嗪青霉素、先锋五号、头孢曲松钠、新霉素中介,对羧苄青霉素、强力霉素、青霉素、氨苄青霉素、红霉素有较高的耐药性.葡萄球菌对先锋霉素V、丁胺卡那霉素敏感,对头孢曲松钠中介,对其他抗生素耐药.其中大肠杆菌对药物敏感率、中介率、耐药率均为33.33%;葡萄球菌的敏感率为13.33%,中介率为6.67%,耐药率为80.00%.%he pathogen were isolated and identified on feces of sick piglets and disease of the spleen, liver, lymph nodes, lung, kidney edema fluidin a pig farm in Hunan northwestern region. Susceptibility of 15 kinds antimicrobial drug was determined with the Kirby-Bauer for isolated strains. The results showed piglets were infected with E. coli and Staphylococcus. Escherichia coli was sensitive to gentamicin, cefuroxime, Amikacin, tetracycline, cefoperazone sodium, to chloramphenicol, piperacillin, pioneer V, ceftriaxone, neomycin was intermediate, to carbenicillin, doxycycline, penicillin, ampicillin, erythro-mycin had a higher resistance. Staphylococcus was sensitive to pioneer V, Amikacin, to ceftriaxone was intermediate, on the other antibiotic was resistance. Which was 33.33% the rate of drug sensitivity, intermediate and resistance to E. coli. Which was 13. 33% sensitive rate, intermediate rate was 6. 67%, resistance rate was 80. 00% to Staphylococcus.

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