Direct-tissue culturing was used to isolate and culture rat epidermal stem cells. Then, observe the appearance of the culturing cells, and the expression of CK15, P63 and α6-integrin in ESCs were detected by immunohistochemistry staining. By the way, the ESCs marked by α6-integrin were detected by flow cytometry CFCM). Meanwhile, the growth curve of epidermal stem cells was also detected. We could isolate, purify and culture rat epidermal stem cells successfully by direct-tissue culturing method and these cells grew well. Immuno-histochemistry staining showed that CK15, P63 and α6-integrin were strongly expressed in the cultured epidermal stem cells. According to the flow cytometry, the probability of Micky cells marked by 06-integrin was 80% , while the probability of unsticky cells marked by a6-integrin was only 2%.%在试验中采用组织块法分离培养大鼠表皮干细胞,观察所培养的细胞形态,免疫组织化学方法检测CK15、P63、α6-integrin表达情况并用α6-integrin标记表皮干细胞经流式细胞仪检测其标记率,同时对1～6代表皮干细胞在不同培养时间计数.结果表明,采用组织块法能分离得到大鼠表皮干细胞,且获得的细胞生长良好；干细胞标记物CK15、α6-integrin、P63呈阳性.经流式细胞仪检测得知,黏附细胞被α6-integrin标记的几率为80％,而未黏附细胞被α6-integrin标记的几率仅为2％,二者相差较大.
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