可乐猪乳头数性状全基因组关联分析

摘要

乳头数性状是猪最重要的繁殖性状之一,与养猪业的经济效益密切相关.本试验以Illunima Porcine SNP 60K芯片对22头可乐猪进行基因分型,通过Plink 1.07软件,基于混合线性模型对左乳头数、右乳头数和总乳头数性状进行全基因组关联分析.经过严格的质量控制和多重检验之后,共鉴定出全基因组水平潜在关联的SNPs位点4个 (P<2.06E-5);染色体水平显著关联位点3个,潜在关联位点18个;在关联SNP位点可能连锁的上、下游1 cM区间内检索到304个基因,其中Wnt及Fgf信号通路中的候选基因BTRC、FGF5、FGF8和BMP3、RASGEF1B、HMGB3可能影响猪的乳头数性状或繁殖性状.通过全基因组关联分析策略发掘出的关联SNP位点及潜在候选基因,将为可乐猪的选育保种奠定基础.%Teats number is one of the most important reproductive traits,and closely related to the economic benefit in pig industry.In order to reveal the underlying genetics of left teats number,right teats number and total teats number traits,a genome-wide association study (GWAS) was performed.Samples of DNA were collected to genotyping for 22 Kele pigs using the Illumina Porcine SNP 60K Chip.The GWAS was performed using a mixed-effects model and linear regression approach.When a genome-wide threshold was determined using the Bonferroni method (P<2.06E-5),4 single nucleotide polymorphism (SNP) markers were potentially associated with left teats number,right teats number and total teat number.However,3 SNPs were significant associated and 18 SNPs were potentially associated in chromosomes level.304 Ensembl genes were retrieved around 1 cM of the associated SNPs.The candidate genes in Wnt and Fgf signaling pathway (BTRC,FGF5,FGF8,BMP3,RASGEF1B and HMGB3) might have effect on target traits.These results provided valuable information about the selective breeding for Kele pigs.