MYNN基因在猪不同组织及肌肉中的表达规律

摘要

试验旨在研究myoneurin(MYNN)基因在猪不同组织中的表达特征及其在肌肉(背最长肌、股二头肌和腰大肌)、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律.采用实时荧光定量PCR技术研究猪MYNN mRNA在90日龄大白猪和马身猪的心脏、肝脏、脾脏、肺脏、肾脏、小脑、小肠、胰脏、胃、股二头肌及脂肪共11个组织中的表达谱,以及在大白猪和马身猪1、90、180日龄3个发育阶段的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律.结果表明,MYNN在猪的各种组织中广泛表达,且各组织间表达差异显著或极显著(P＜0.05;P＜0.01);MYNN在大白猪和马身猪的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的不同发育阶段表达差异显著或极显著(P＜0.05;P＜0.01),并具有特定规律,由此推测其可能在猪的这几种组织中发挥重要作用.MYNN基因的表达与组织、日龄及品种的遗传背景有关.本试验为研究猪MYNN基因的生物学功能提供了依据,但还需要深入的研究来探索其作用的具体机制,尤其是在骨骼肌发育中的调节机制.%This study was aimed to investigate the expression characteristics of myoneurin (MYNN) gene in different tissues and its developmental expression in muscles (longissimus dorsi,biceps femoris and psoas major),cerebellum,liver,pancreas,kidney,stomach,spleen and lung tissues of pigs.The expression characteristics of MYNN mRNA in 11 different tissues including heart,liver,spleen,lung,kidney,cerebellum,small intestine,pancreas,stomach,biceps femoris and fat of Large White pigs and Mashen pigs at the age of 90 days and the developmental expression patterns in muscle,cerebellum,liver,pancreas,kidney,stomach,spleen at three strages (1,90,180 days) of Large White pigs and Mashen pigs were studied by Real-time PCR.The results showed that MYNN was widely expressed in various tissues of pigs,and there was significant difference among the tissues(P＜0.05;P＜0.01);The expression of MYNN in muscle,liver,pancreas,cerebellum,kidney,stomach,spleen,lung tissues were significant difference at three development stages of Large White pigs and Mashen pigs(P＜0.05;P＜0.01),and also had a specific rule,which indicated that it may play an important role in these pig tissues.The expression of MYNN gene could related to the tissue,age and the genetic background of breeds.The results of this study provided a better understanding of the biological functions of pig MYNN.Further studies are required to determine its molecular mechanisms,especially in the regulation of skeletal muscle development.