猪嗜淋巴细胞疱疹病毒2型GD27株gB基因生物信息学分析

摘要

试验采用 PCR 方法扩增出猪嗜淋巴细胞疱疹病毒2型（porcine lymphotropic herpesviruses 2，PLHV-2） gB 基因全序列，并进行测序，使用生物信息学软件对 gB 蛋白进行生物信息学分析和预测。结果显示，gB 蛋白由876个氨基酸组成，分子式为 C4500 H6975 N1199 O1351 S40，分子质量为100．77 ku，等电点为6．45，不稳定系数为38．58；二级结构预测以无规则卷曲为主要结构，1－39位氨基酸为信号肽，761－780位氨基酸为跨膜区结构，具有多种功能位点，存在多处天然非规则结构蛋白；三级结构显示有一个具有溶解作用的环状构象。综合多种方法分析预测结果表明，gB 基因存在多个 B 细胞线性抗原表位，可作为 PLHV-2疫苗的候选抗原。%The glycoproteins B (gB)gene of porcine lymphotropic herpesviruses 2 (PLHV-2)was amplified by PCR and sequenced,then bioinformatics analysis were conducted.The gB protein was composed of 876 amino acids,the molecular formula was C4500 H6975 N1199 O1351 S40 ,the molecu-lar mass was 100.77 ku,the value of theoretical isoelectric point was 6.45,and the instability in-dex was 38.58.The prediction of secondary structure revealed that some alpha helixes and beta sheets exist in protein while random coil was major pattern.It had a signal peptide in the position 1-39 amino acids and a transmembrane helice in the position 761-780 amino acids.It contained several prosites and several intrinsically disordered proteins.Tertiary structure model of gB pro-tein showed a fusion loop.Multiple antigenic epitope located in gB through comprehensive analy-sis prediction method.It was suggested that gB gene could be as a candidate antigen for vaccina-tion of PLHV-2.