The study was aimed to establish a stable model of acute ulcerative colitis(UC)in dogs using acetic acid.Sixteen healthy dogs were randomly divided into four groups:Control group and three acetic acid(with different concentrations)groups.After anesthetic Quanmianbao injection, the acetic acid groups was perfused with 4%,7% and 10% 2 mL/kg acetic acid,respectively.The control group was not anesthetized and the rectal perfusion was given the same dose of sterile sa-line.The defecation and the colorectal mucosa were observed by electronic endoscopy at the 1st, 3rd and 7th days after injection.All the experimental dogs were sacrificed on the 7th day.The le-sioned tissues were collected and examined by histological.The results showed that:In 4% acetic acid group,short-term dilute feces and mild inflammatory cells infiltration was observed.In 10%acetic acid group,dogs died because of strong inflammation w hich lead to colon perforation.In 7%acetic acid group,both fecal traits and colon endoscopy check showed significant symptoms of co-litis,and ulcer foci formed and droped off after 3 and 7 d of acetic acid administration,respective-ly.Histological examination showed that there was significant inflammatory cells infiltration around the ulcer foci,and it would significantly reduced as time goes on,and replaced by fibroblast hyperplasia.In conclusion,acute ulcerative colitis in dogs could be successfully induced by 7%acetic acid perfusion.%试验旨在探索醋酸诱导犬急性溃疡性结肠炎(ulcerative colitis,UC)的方法,建立稳定的犬急性UC模型.选取16只健康犬,随机分成对照组和3个不同浓度醋酸组,每组4只.注射犬眠宝麻醉后,醋酸组分别灌注4%、7% 和10% 的2 mL/kg醋酸,对照组无需麻醉,直肠灌注相应剂量无菌生理盐水;用药后第1、3、7天观察各组排便情况,并用电子内窥镜观察试验犬结肠黏膜的损伤程度;于第7天牺牲试验犬,切取病变结肠做组织切片检查.结果显示,灌注4% 醋酸的犬均出现短暂的稀便,病理切片观察只有轻微炎性细胞浸润;灌注10% 醋酸的犬,炎症反应过于强烈导致肠穿孔和死亡;灌注7% 醋酸的犬,粪便性状及结肠内窥镜检查均显示明显的结肠炎症状,3d后出现溃疡灶,7d后可见溃疡灶脱落,组织学检查发现溃疡灶周围有明显的炎性细胞浸润,随着时间的推移炎性细胞浸润明显减少,成纤维细胞增生.以上结果表明,直肠灌注7% 醋酸能成功稳定诱导犬急性UC.
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