紫锥菊多糖对LPS损伤后IEC-6细胞分泌IL-1ɑmRNA的影响

摘要

为了研究紫锥菊多糖对LPS损伤后IEC-6细胞分泌IL-1αmRNA的影响，以体外培养大鼠小肠上皮细胞为模型，进行细胞分组及收集，并给予LPS刺激造成内毒素损伤，根据GenBank中发布的IL-1ɑ基因序列设计引物，以基因组RNA为模板进行RT- PCR扩增，发现紫锥菊多糖对LPS刺激小肠上皮细胞产生的IL-1αmRNA的表达减少。%To study the effect of Echinacea polysaccharide on the secretion of IL - 1α mRNA of damaged IEC-6 cell. In cultured rat intestinal epithelial cells as a model, we are to grouping and collecting the cells and stimulated LPS with endotoxin to cause damage. According to the GenBank IL-1ɑ gene primers released, we used Genomic RNA as the template for RT-PCR amplification and found that the expression of Echinacea polysaccharide on IL-1αmRNA stimulated by LPS on intestinal epithelial cells was decreased.