狂犬病毒ELISA抗体检测试剂盒的初步应用

摘要

5 batches of rabies virus ELISA antibody detection kits were produced by Ringpu (Baoding) biological pharmaceutical company limited, with the inactivated and purified RABV SRV- 9 strain as coating antigen. Parallel testing on 87 clinical serums between the kit and FAVN, imported kit of the same type showed the total accordance rates being 90.80%and 94.25%, respectively. And the coefficients of variation of inter- batch and intra- batch kits were 1.47%~8.34% and 2.05%~8.64%, respectively. The kit was simple, sensitive, specific and suitable for he detection of the positive rabies virus antibody in serum in scale.%以灭活纯化的狂犬病毒SRV9株作为包被抗原，制备了5批次狂犬病毒ELISA抗体检测试剂盒中试产品。与FAVN法、商品化进口试剂盒相比，该试剂盒与二者总符合率为90.80%和94.25%，且敏感性较高。5批次试剂盒批内变异系数为1.47%~8.34%，批间变异系数为2.05%~8.64%，说明该试剂盒重复性良好，可用于血清中狂犬病毒抗体常规检测。