蒙古马高负荷运动训练前后miRNA差异表达分析

摘要

A total of 6 Mongolian horses after 4 months high-load training were taken as study object in order to discover differentially expressed genes and related metabolic pathways.The muscle samples of these horses were respectively collected before and after the training.By using next generation sequencing technology,a miRNA library was established.Target genes were then predicted and enriched through GO analysis,and analyzed by KEGG pathway.The results showed that:1) Before and after the high load training,366 and 346 miRNAs were identified respectively.A total of 216 co-expressed miRNAs were identified and 7 620 and 7 535 target genes were predicted.The expression abundances of differentially expressed miRNAs were miR-1,miR-378,miR-21,miR-101 and miR-133a;2) The GO enrichment results showed that miRNA's target genes were annotated to biology processes including developmental process,anatomical structure development,protein binding,enzyme binding,etc.The KEGG analysis results displayed that the target genes were annotated to 210 pathways including actin cytoskeleton metabolic,calcium signaling pathways,cardiac muscle contraction,etc.This study provided theoretic support for further investigation of the molecular mechanism of Mongolia horse strong stamina and offered new insights for comprehensively understanding Mongolian horse's athletic characteristics.%为研究蒙古马高负荷运动训练前后miRNA差异表达,利用二代测序技术对6匹经4个月高负荷运动训练的蒙古马进行了调查,筛选训练前后发生差异表达的相关基因及其所参与的生物学过程.在训练前与训练后两个时期分别采集肌肉样品,建立蒙古马肌肉miRNA文库,对训练前后表达量发生显著变化的差异miRNA进行靶基因预测,并进行GO功能富集与KEGG Pathway分析.结果表明:1)在蒙古马高负荷训练前后两个文库中,分别筛选得到366和346个miRNA,共表达miRNA达248种,分别预测得到7 620和7 535个靶基因.其中高丰度表达的miRNA有miR-1、miR-378、miR-21、miR-101、miR-133a等;2)靶基因参与调控的生物学过程包括:发育过程、解剖结构发育、蛋白质结合、酶结合等;3)KEGG Pathway结果显示,在210条被注释的信号通路中与运动代谢相关的有:调节肌动蛋白细胞骨架,钙信号通路、心肌收缩等.本研究结果可为探究蒙古马强耐力的基本分子机理提供理论基础,同时也为了解蒙古马运动学特性提供新思路.