蒙古马高负荷运动训练前后转录组差异表达分析

摘要

旨在对蒙古马高负荷运动训练前后转录组差异表达进行分析.本试验对6匹进行了4个月高负荷运动训练的蒙古马进行研究,分别在训练前后两个时期采集肌肉样品,利用二代测序技术对肌肉样品建立转录组文库,对发生显著变化的差异基因进行GO功能富集和KEGG Pathway分析,寻找训练前后发生显著变化的代谢通路及相关候选基因.结果表明,在转录组筛选到的1 102个显著差异表达基因中,有299个在训练后发生下调,803个发生上调.这些差异基因被注释到3 398条GO term上.其中与运动学相关的生物学过程有:肌肉结构发育、心血管系统发育、循环系统发育、肌肉组织发育、肌肉器官发育、肌肉收缩和肌肉细胞分化等.对差异表达基因进行KEGG pathway分析,结果表明,差异基因被富集到如扩张型心肌病、肥厚型心肌病、心肌收缩、钙信号通路、肌动蛋白骨架调节等与运动相关的193条通路中.本研究结果为探究蒙古马强耐力的基本分子机理提供理论基础,同时也为理解蒙古马运动学特性提供新的思路.%The aim of this study was to analyze the Mongolian horse transcriptome differential expression before and after a high load exercise training.This research selected 6 Mongolian horses with high load training for 4 months.Muscle samples were collected before and after the training respectively.The transcriptome library was established using the second generation sequencing technology.We enriched the differentially expressed genes to the GO and KEGG pathway,and screened the significantly different metabolic pathways and related genes.1 102 differentially ex pressed genes(DEGs) in transcriptome library were screened out,of which 299 were down-regulated and 803 were up-regulated after high load training,respectively.These DEGs were annotated to 3 398 GO terms,including muscle structure development,cardiovascular system development,circulatory system development,muscle tissue development,muscular organ development,muscle contraction,muscle cell differentiation,etc.Also the DEGs were annotated to 193 KEGG pathways including some athletics related pathways:dilated cardiomyopathy (DCM),hypertrophic cardiomyopathy,cardiac muscle contraction,calcium signaling,the actin cytoskeleton adjustment,etc.Our findings provide a theory support for further investigating the molecular mechanism of Mongolian horse strong stamina and a new research method for Mongolian horses athletic characteristics.