肠杆菌Enterobacter sp.S8共代谢生物降解邻苯二甲酸二丁酯的特性研究

摘要

邻苯二甲酸二丁酯(DBP)是目前应用最为广泛的邻苯二甲酸酯之一,属于难降解有机污染物,具有较强的内分泌干扰性.本文以分离得到的DBP降解菌Enterobacter sp.S8为研究对象,系统考察了共代谢基质类型和共代谢反应条件对S8共代谢生物降解DBP的影响,同时对其反应动力学及酶的稳定性进行了研究.结果表明,在无共基质添加的条件下,S8对DBP的降解率仅为30.2％,而在温度为30℃、pH为7、甲醇投加量为20mg/L以及DBP初始浓度为300mg/L的优化共代谢降解条件下降解率达到最大,为75.6％;其中DBP初始浓度对共代谢降解效果影响最大.本文的结果为采用生物共代谢方式进行DBP污染环境的治理提供了依据.%Dibutyl phthalate (DBP) is one of the most widely used phthalates,which is refractory organic pollutants and has strong endocrine disrupting effect.In the present study,the isolated DBP degrading bacteria,Enterobacter sp.S8 was applied to investigated the effects of the co-metabolism substrate type and reaction conditions on the co-metabolism degradation efficiency of DBP,while the reaction kinetics and the stability of the enzyme were studied.The results showed that the biodegradation efficiency of S8 to DBP was only 30.2％ without co-metabolism substrate,while the highest DBP biodegradation efficiency was 75.6％ with optimal conditions:the temperature of 30℃,the pH of 7,the methanol dosage of 20 mg/L and the DBP initial concentration of 300 mg/L.The initial concentration of DBP had the greatest effects on the co-metabolism biodegradation of DBP.The present study provides a basis for the treatment the DBP contaminated environments by co-metabolism.