生物处理纯化玉米芯半纤维素糖液的研究

摘要

Biological treatment was used to remove glucose and galactose in this paper to purify the hemicellulose liquid sugars. Single factor and central composite experiments were used to optimize the medium components in the biological treatment. It was seen that the optimal results were obtained when 5. 7 g/L (NH4)2SO4, 2. 0 g/L KH2PO4 and 2. 4 g/L yeast were applied. The removal of glucose and galactose were 100% and 67%, and the purity of xylose increased from 47% to 60% after the optimal biological treatment. Acclimatization of strain used in the biotreatment of the liquor was also conducted to handle the high sugar consistency liquor which was diluted 6 times.%通过采用生物法处理以消除半纤维素糖液中的葡萄糖、半乳糖等杂糖组分,达到纯化半纤维素糖液,提高木糖含量的目的,可望有效提高木糖的收率。采用单因素试验和中心组合实验优化了玉米芯半纤维素糖液在生物处理时的最佳培养基组分(g/L)为：(NH4)2SO45.7、KH2PO42.0、酵母粉2.4。将木糖母液稀释10倍,在此最佳组分下,采用生物处理24 h,半纤维素糖液中的葡萄糖和半乳糖去除率分别达到100%和67%,木糖质量分数从47%提高到60%。通过进一步对菌株进行逐步驯化,使其可处理仅稀释6倍的高浓度糖液。