竹叶总黄酮对脂肪酸合酶及人乳腺癌细胞的抑制作用

摘要

The inhibitory effects of bamboo leaves flavonoids ( BLF) from the species of Pleioblastus amarus ( Keng) keng, Indocalamus latifolius (Keng) McClure, Phyllostachys heterocycla (Carr.) Mitford Pubescens and P.glauca McClure on the fatty acid synthase (FAS) and human breast cancer cell MDA-MB-231 in vitro were studied.The inhibitory effects of bamboo leaves flavonoids on FAS were determined by UV spectrophotometry , and the inhibitory effects of MDA-MB-231 were detected by CCK-8 assay.Flow cytometry was used to detect the apoptosis of MDA-MB-231.The results showed that all four bamboo leaves flavonoids had significant inhibitory effects on FAS and MDA-MB-231 cells.The IC50 values of four flavonoids against FAS were 112.43, 153.29, 161.32 and 195.09 mg/L, respectively.The inhibition effects of flavonoids on MDA-MB-231 followed the dose-effect relationship .Further studies showed that the 70%and 95%ethanol fraction of bamboo leaves flavonoids exhibited the strongest cytotoxicity .The inhibition rates against MDA-MB-231 at the mass concentration of 100, 200, 400 mg/L of 95%ethanol fraction of P.amarus were 25%, 44%and 70%, and the apoptosis rates were 11.1%, 23.1%and 38.7%, respectively.%研究了毛竹、苦竹、阔叶箬竹、淡竹竹叶总黄酮对脂肪酸合酶(FAS)和人乳腺癌细胞(MDA-MB-231)的体外抑制活性.通过紫外分光光度法测定竹叶总黄酮对FAS的抑制作用,CCK-8法检测MDA-MB-231细胞的体外增殖抑制作用,流式细胞仪检测MDA-MB-231细胞凋亡作用.结果表明:毛竹、苦竹、阔叶箬竹、淡竹竹叶总黄酮粗提物对FAS抑制活性显著,其IC50依次为:112.43、153.29、161.32和195.09 mg/L;4种竹叶总黄酮粗提物对MDA-MB-231细胞具有显著体外抑制活性,且具有良好的量效关系.竹叶总黄酮的主要活性部位为大孔树脂70%和95%乙醇相;苦竹竹叶95%乙醇相对MDA-MB-231细胞抑制活性最强,当质量浓度100、200、400 mg/L时对MDA-MB-231细胞增殖抑制率分别为25%、44%和70%;凋亡率分别为11.1%、23.1%和38.7%.