SPA had high-level expression in E.coli BL21 with pET32a(+)as expression plasmid vector in M9 medium under the conditions as follows:temperature was 15℃ ,IPTG concentration was 0. 8 mmol·L-1, rotation speed was 150r·min-1 .induction time was 4 h. The coupling rate of SPA was 89.67% and it had the best purification efficiency for igG when the coupling time was 14 h and the proportion of SPA and Sepharose 4B was 1:1(mass-volume ratio).%使用M9培养基,以大肠杆菌BL21为宿主菌、pET32a(+)为载体,在15℃、IPTG浓度为0.8 mmol·L-1、150 r·min-1、诱导时间为4d的条件下,SPA能够得到高效表达；在SPA:Sepharose 4B=1:1(质量体积比)、偶联时间为14 h时,SPA偶联率为89.67％,对IgG的纯化效果最好.
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