产纤维素酶细菌的筛选及其紫外诱变

摘要

采用刚果红脱色圈法初筛得到143株有纤维素酶活性的细菌,然后采用DNS法复筛得到纤维素酶酶活最高的6株细菌,进一步进行紫外诱变处理,获得酶活提高最大且具有遗传稳定性的菌株E140',酶活为0.90 IU· mL-1,较出发菌株(0.68 IU·mL-1)提高了32.35％.表明采用刚果红脱色圈法和DNS法联合筛选并结合紫外诱变,可以获得纤维素酶活性高的细菌.%Firstly, 143 strains with cellulase activity were obtained by primary screening with Congo red decolorizing ring method,and then 6 strains with the highest enzyme activity were obtained by screening with DNS method. After the furtherly UV mutagenesis treatment,a mutant strain E140' with the highest enzyme activity increasing and genetic stability was got, whose enzyme activity reached 0. 90 IU · mL-1,increasing by 32. 35% .than that of the starting strain(0. 68 IU · mL-1). It showed that cellulase-producing bacteria with high enzyme activity could be obtained by screening with both Congo red decolorizing ring method and DNS method, combining with UV mutagenesis treatment.