Using tobacco as raw material,with the extraction rate of essential oil and neophytadiene,the quality of essential oil as evaluation indexes,the effects of volume fraction and flux of modifier,extraction pres-sure,extraction temperature,supercritical CO2 fluid flux,extraction time on extraction efficiency were investiga-ted by single factor experiment.Based on the results obtained,the optimum process parameters were determined by orthogonal experiment as follows:with 94% ethanol at the flux of 0.04 mL·min-1 as modifier,extraction pressure was 25 MPa,extraction temperature was 50 ℃,supercritical CO2 fluid flux was 2.0 L·min-1 ,extrac-tion time was 3.0 h.Under the optimal conditions,the essential oil was golden in color and had fewer residues, the extraction rate of essential oil and neophytadiene was 37.58 mg·g-1 and 4.045 mg·g-1 ,respectively.%以烟草为材料,以精油提取率、新植二烯提取率、精油品质为评价指标,通过单因素实验考察夹带剂乙醇的体积分数、夹带剂流量、萃取压力、萃取温度、CO2流量、萃取时间等因素对萃取效果的影响。在此基础上,通过正交实验优选萃取工艺条件为:94%乙醇为夹带剂、夹带剂流量0.04 mL·min-1、萃取压力25 MPa、萃取温度50℃、CO2流量2.0 L·min-1、萃取时间3.0 h,在此条件下萃取的精油金黄透亮,夹带剂残留少,精油提取率为37.58 mg·g-1,新植二烯提取率为4.045 mg·g-1。
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