金纳米粒的绿色合成及其在铜离子检测中的应用

摘要

The fluorescent gold nanoparticles(AuNPs)were green synthesized by reduction of chloroauric acid with modified yeast cells as stabilizer and reducing agent in the presence of sodium hydroxide.AuNPs were confirmed by X-ray diffraction and UV-visible spectroscopy.A simple and rapid detection method of Cu2+ in food products was developed based on the fluorescence quenching of the as-prepared AuNPs induced by Cu2+. Results indicated that,the quenched fluorescence of AuNPs was linearly proportional to the concentration of Cu2+ in the range from 0.001 μg·mL-1 to 0.2 μg·mL-1 ,the accuracy and precision of the developed method met the requirements of food analysis,and had been successfully applied to the detection of Cu2+ in rice and dried tofu.%以改性酵母细胞为稳定剂和还原剂，在氢氧化钠存在下通过原位还原氯金酸绿色合成了荧光金纳米粒（AuNPs），并对其进行了 X-射线衍射和紫外光谱表征。基于 Cu2＋对 AuNPs 的荧光猝灭作用，建立了一种快速简便地检测食品中微量 Cu2＋的方法。结果表明，Cu2＋浓度在0.001～0.2μg·mL－1范围内，AuNPs 的荧光猝灭程度与 Cu2＋浓度间呈良好的线性关系。方法的准确度和精密度满足食品分析的要求，已成功用于大米和豆干中微量 Cu2＋的测定。