复杂生物样品中马来酸依那普利的快速分析新方法

摘要

A method for fast determination of enalapril maleate in complex biological matrices was devel-oped.The content of enalapril maleate in plasma,saliva and urine matrices were directly and rapidly determina-ted by HPLC-DAD combined with the second-order correction algorithm of SWATLD.The average recovery of enalapril maleate in three biological matrices of plasma,saliva and urine were (98.0±3.7)%,(98.1 ±6.0)%and (100.8±3.4)%,respectively.The performance of the method was evaluated by analyzing the quality fac-tors,including sensitivity(SEN),selectivity(SEL),limit of detection(LOD),limit of quantitation(LOQ)and RMSEP.t-Test was used to check the results.This method had the “second-order advantage”.Enalapril mal-eate was distinguished efficiently and analyzed quantitatively in the presence of different biological matrixs with endogenous substances,and the analytical results were accurate and reliable.%建立了复杂生物基质中马来酸依那普利的快速检测新方法。运用高效液相色谱-二极管阵列（HPLC-DAD）检测技术结合自加权交替三线性分解（SWATLD）二阶校正算法直接快速测定血浆、唾液和尿液生物基质中马来酸依那普利的含量，获得血浆、唾液和尿液3种生物基质中马来酸依那普利的平均回收率分别为（98．0±3．7）％、（98．1±6．0）％和（100．8±3．4）％。通过分析品质因子，包括灵敏度（SEN）、选择性（SEL）、检测限（LOD）、检测量（LOQ）和预测均方差（RMSEP）评估了该方法的性能，利用 t-检验对结果进行了校验。结果表明，该方法具有的“二阶优势”可在不同生物基质样中内源化学物质干扰共存下，有效分辨和定量分析马来酸依那普利，结果准确可靠。