紫外-ARTP复合诱变选育达巴万星前体高产菌株

摘要

Dalbavancin precursor-producing strain Nonomuria spp .DW-3-19 was breeded by UV-atmosphere-ic and room temperature plasma(ARTP) composite mutagenesis and streptomycin-resistant mutant screening . The results showed that a high-yield strain possessing stable genetic performance could be obtained using the composite mutagenesis ,and the transformation efficiency was increased by 68 .7% over the starting strain .The results also show that ARTP mutagenesis can be effectively used for the mutagenic breeding of Actinomycetes . UV-ARTP composite mutagenesis can improve the fermentation unit of dalbavancin precursor ,reduce produc-tion cost ,and obtain the high-yield strain with stable genetic performance in a short time.%采用紫外-常压室温等离子体(ARTP)技术对达巴万星前体产生菌野野村放线菌属(Nonomuria spp.)菌株DW-3-19进行复合诱变,并进行链霉素抗性突变选育,获得了具有稳定遗传性的高产菌株,该菌株发酵单位比出发菌株提高了68.7%.表明,ARTP技术可有效应用于放线菌的诱变选育,紫外-ARTP复合诱变可提高达巴万星前体发酵单位,降低生产成本,为短时间内获得稳定遗传的高产菌株提供了可行的方法.