忍冬根的正丁醇萃取物化学成分研究

摘要

We separated six high purity monomers from n-butanol extract of Lonicera j aponica Thunb.roots by reversed phase C18column chromatography,silica gel column chromatography,and preparative high perform-ance liquid chromatography.Moreover,we characterized their chemical structures by nuclear magnetic resonance (NMR)and electrospray ionization mass spectrometry,and identified as chlorogenic acid(Ⅰ),sweroside(Ⅱ), loganin(Ⅲ),secoxyloganin(Ⅳ),4-O-(4′-O-caffeoyl glucosyl)-5-O-caffeoylquinic acid(Ⅴ),and 3,5-O-dicaf-feoylquinic acid(Ⅵ),respectively.In which,loganin(Ⅲ)is firstly isolated from Lonicera j aponica Thunb. roots,and 4-O-(4′-O-caffeoyl glucosyl)-5-O-caffeoylquinic acid(Ⅴ)is firstly isolated from Lonicera plants.The study lays the foundation of further exploitation and utilization of Lonicera plants and further understanding of medicinal active ingredients.%采用反相C18柱色谱、硅胶柱色谱、制备型高效液相色谱成功地从忍冬根的正丁醇萃取物中分离得到6个高纯度单体,通过核磁共振波谱和质谱鉴定其为:绿原酸(Ⅰ)、獐芽菜苷(Ⅱ)、马钱苷(Ⅲ)、断氧化马钱子苷(Ⅳ)、4-O-(4′-O-咖啡酰葡萄糖基)-5-O-咖啡酰奎宁酸(Ⅴ)、3,5-O-二咖啡酰奎宁酸(Ⅵ).其中,马钱苷(Ⅲ)为首次从忍冬根中分离得到,4-O-(4′-O-咖啡酰葡萄糖基)-5-O-咖啡酰奎宁酸(Ⅴ)为首次从该种属植物中分离得到.该研究为进一步开发利用忍冬植物资源,深入了解其药用活性成分奠定了基础.