活体内五氯苯酚(PCP)的检测，往往是通过检测其代谢产物实现.四氯苯醌(TCBQ)作为 PCP 的一种主要代谢产物，发展其检测方法具有重要意义.该文基于 TCBQ 与 ssDNA 的 G 碱基发生特异性化学反应，破坏 ssDNA 的结构，从而破坏 ssDNA 对金纳米粒子的保护作用，导致金纳米粒子在盐诱导下聚沉发生颜色改变.通过测定 ssDNA 保护的金纳米粒子溶液颜色的改变，实现 TCBQ 的定量检测.该检测方法线性范围宽(9.3 nmol/L~0.93 mmol/L)，检测限达到了3 nmol/L.干扰实验表明该方法选择性高% The detection of PCP in vivo is generally realized through the detection of tetrachloro-p-benzoquinone (TCBQ), a metabolite of PCP. As one of the major metabolite of PCP, it is of great interests to develop its detection techinque. In this work, a label-free colorimetric detection of TCBQ is developed based on the specific reaction between TCBQ and G bases of ssDNA. The TCBQ-ssDNA reaction changes the structure of ssDNA, leading to the destroy of the ssDNA-protected gold nanoparticles (NPs), and cosequently the salt-induced aggregation of Au NPs. TCBQ was detected based on the TCBQ-ssDNA reaction-resulted change of the Au NPs solution. The proposed method exhibits a wide linear range of 9.3 nmol/L ~0.93 mmol/L, and a high sensitivity with a limit of detection (LOD) of 3 nmol/L. Interference experiments demonstrate this method is with a good selectivity.
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