根皮苷分子印迹聚合物制备及其识别性能研究

摘要

为了制备对根皮苷具有仿生识别能力的分子印迹聚合物,利用本课题组所建立的紫外光谱法筛选并确定了制备根皮苷分子印迹聚合物所需的功能单体(4-乙烯基吡啶)及其与模板分子间的最佳比例(6:1).然后以根皮苷为模板分子,4-乙烯基吡啶为功能单体,乙二醇二甲基双丙烯酸酯为交联剂,合成了根皮苷分子印迹聚合物.经研磨、过筛、索氏提取去除模板分子后得到相应的分子印迹聚合物颗粒.采用静态平衡吸附实验研究了该分子印迹聚合物对根皮苷的结合与识别能力.结果表明与化学组成相同的非印迹聚合物相比,根皮苷分子印迹聚合物对根皮苷具有较好的识别性能.%In order to prepare molecularly imprinted polymer ( MIP ) for phlorizin with biomimetic rec-ognition ability, functional monomer and proportion selection method established by our group was used to select the functional monomer (4-vinyl pyridine) and optimal proportion (6 : 1) suited for phlori-zin. Then, using phlorizin as template, 4-vinyl pyridine as functional monomer and ethylene glycol dimethacylate as cross-linker MIPs for phlorizin was prepared. After grinding, screening and Soxhlet extraction, MIPs particles were obtained. Using equilibrium binding experiments, the recognition per-formance of MIPs particles to phlorizin was studied. The results show that the MIPs particles exhibited much better affinity towards phlorizin than non-imprinted polymers particles.