A new method was developed for the analysis of the volatile and semi-volatile acidic components in tobacco by gas chromatography-mass spectrometry selected ion monitoring (GC-MS, SIM)method. The acidic components in tobacco samples were extracted by methylene chloride using simultaneous distillation and extraction equipment, and they were analyzed by HP-INNOWAX column (30m × 250μm × 0.25μm ). Thirteen acidic components were quantitatively determined by internal standard curve method. The experiment results showed that the added standard recoveries of the acidic components were in the range from 80.6% to 98.8%, the relative standard deviations(RSD)were less than 2.0% and the correlation coeflqcients were more than 0.99. The method is simple, rapid and accurate for the determination of the volatile and semi-volatile acidic components in tobacco.
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