双波长共振散射比率法测定十二烷基苯磺酸钠的研究

摘要

基于表面活性剂SDBS与罗丹明B的结合反应发展了一种测定SDBS的双波长共振散射比率法.在BR缓冲溶液介质中,SDBS能与RB发生反应,其位于373.0nm的特征共振光散射信号大大增强.通过分别测定I373.0和Ⅰ685.0/I650.0来检测SDBS,当罗丹明B的浓度为1.0×10-4 mol/L时,以Ⅰ373.0为测定点的共振光散射方法所得到的线性范围和检出限分别为0.018～80 μmol/L和1.76 nmol/L.而用I685.0/I650.0的散射强度之比来检测SDBS时,其得到的线性范围和检出限分别为0.002～100 μmol/L和0.23 nmol/L.表明后者即双波长共振散射比率法明显优于前者.%A dual-wavelength resonance lighting scattering(DW-RLS)ratio method is developed to detect surfactant SDBS based on its bindings with Rhodamine(RB). In Britton - Robinson buffer controlled medium,SDBS could interact with RB,displaying significantly enhanced RLS signals. By measuring the RLS signals characterized at 373. 0 nm(I373.0)sind the RLS intensity ratio(I685.0/ I650.0) .respectively,SDBS over a wide dynamic range of content could be detected. Typically,when RB concentration is kept at 1.0 x10-4 mol/L,using I373.0 could detect SDBS over the range of 0.018-80μmol/L with the limit of 1.76 nmol/L,while using I685.0/I650.0 could detect SDBS over the range of 0.002-100μmol/L with the limit of 0.23 nmol/L Showing the latter so-called DW-RLS ratio method is obviously superior to the former one.