新型荧光增强法检测水样品中碘离子

摘要

Hg2+could quench the fluorescence of riboflavin ,and iodine ion(I-)could react with I-to form HgI2 or HgI2.On the basis of these two phenomena ,a novel“turn-on”fluorescent method for the decetection of I -was developed in the present work.In this strategy,the fluorescence of riboflavin at the wavelength of 525 nm was quenched owing to the addition of Hg 2+.While in the pres-ence of different concentrations of I -,I-could react with Hg 2+and make the fluorescence of riboflavin and Hg 2+mixture“turn-on”in-creasingly.This“turn-on”fluorescent method had linear relationship with I -from 1 to 100μmol· L-1 with the limit of dectect ( LOD) of 0.4μmol· L-1.Furthermore,this fluorescent method had fast reponse time to I -(2 min),good slectivity of other haloid ions and good recoveries in the anlysis of I-in drinking water .%Hg2＋可以猝灭核黄素的荧光，碘离子（ I-）可以与Hg2＋形成HgI2，基于此，发展了一种新型的荧光增强法检测水中I-的方法。 Hg2＋加入后，核黄素在525 nm处的荧光被猝灭；继续在体系中加入不同浓度的I-，体系中Hg2＋被不断消耗，导致核黄素在525 nm处的荧光逐渐增强。该方法检测I-的线性范围在1-100μmol· L-1，检测限是0.4μmol· L-1；同时，该方法具有较快的响应时间（2 min）；对其他卤素离子具有较好的选择性；用于水中I-检测时得到了较好的回收率。