Ag（III）-鲁米诺化学发光体系对泼尼松龙的测定研究

摘要

A new method was established to detect prednisolone based on a new system of Ag( III) complexes-luminol chemilumines-cence(CL). In alkaline medium,prednisolone can change the optical signals of Ag(III)complexes-luminol chemiluminescence sys-tem. Under optimum conditions,It was presented a linear relationship between the prednisolone concentration and the decrease of optical signal. Under optimum conditions,the linear ranges are 2. 0 × 10-9 ~1. 0 × 10-7 mol·L-1 and 2. 0 × 10-7 ~1. 0 × 10-6 mol·L-1 . The detection limit of the method is 5. 0×10-10mol·L-1. Detecting the 2. 0×10-8mol·L-1 and 6. 0×10-7mol·L-1 of prednisolone for 11 times,its relative standard deviations are 1. 1%and 1. 5%,respectively. The recovery of prednisolone acetate injection by this method is 98. 0% ~101%. The method is fast,simple and accurate. It is suitable for the study on the pharmacokinetics of the new formulation of prednisolone,and it can also be used to test other dosage forms of prednisolone.%建立了应用Ag(Ⅲ)配合物-鲁米诺化学发光体系检测泼尼松龙的方法。在碱性介质中,泼尼松龙可以改变Ag(Ⅲ)配合物-鲁米诺化学发光体系的光信号,一定范围内,其浓度与光信号的减弱程度呈线性关系。于最优条件下,该方法的线性范围是2．0×10-9~1．0×10-7 mol·L-1和2．0×10-7~1．0×10-6 mol·L-1,检出限为5．0×10-10mol·L-1。对2．0×10-8mol·L-1与6．0×10-7mol·L-1的泼尼松龙进行平行测定11次,其相对标准偏差分别为1．1%与1．5%。该方法用于醋酸泼尼松龙注射液的测定,回收率为98．0%~101%。本方法具有快速、简便、准确的特点,适用于泼尼松龙新剂型药代动力学的研究,也可应用于泼尼松龙其他剂型的检测。