牛血红蛋白催化荧光光度法测定痕量过氧化氢

摘要

A new fluorometric method is proposed for the determination of trace H2O2. In the concentration of 1. 0×10-4mol·L-1 sul-furic acid solution,it is found that hemoglobin can catalyze benzoic acid to produce hydroxybenzoic acid,which has strong fluores-cent in alkaline solution. At the length of maximum excitation 295 nm and maximum emission 408 nm,the proposed method presents wide linear responses between the added value of fluorescence intensity and H2 O2 concentration in the range from 7. 880 × 10-8 to 1. 182×10-4mol·L-1 with a detection limit of 5. 26×10-8mol·L-1. The regression equation isΔF=7. 418×106 c+9. 742 with a corre-lation coefficient of 0. 9994. The present method has been applied for the determination of H2 O2 in rainwater and disinfectant fluid samples with satisfactory results.%在1．0×10-4mol·L-1的H2SO4中，牛血红蛋白催化H2O2氧化苯甲酸生成羟基苯甲酸，羟基苯甲酸在碱性溶液中有强荧光，据此提出了一种测定痕量H2 O2的方法。在激发波长295 nm与发射波长408 nm处，反应体系的荧光增加值ΔF与H2O2的浓度在7．880×10-8~1．182×10-4mol·L-1范围内存在良好的线性关系。线性回归方程为：ΔF=7．418×106 c+9．742(c的单位为mol·L-1)，相关系数r=0．9994。检出限为5．26×10-8mol· L-1。将方法用于雨水及消毒液中H2 O2的测定，结果满意。