以石墨烯/二氧化锰分散液滴涂在处理好的玻碳电极表面,然后将1-辛基-3-甲基咪唑六氟磷酸盐电聚合制得OMIMPF6/GR/MnO2/GCE电极.利用该修饰电极对黄嘌呤(XA)和尿酸(UA)混合物进行同时分析.结果表明,在0. 2 mol·L-1的磷酸缓冲液(pH＝5. 0)支持电解液中,XA和UA在修饰电极上有较好的电化学行为.在最佳条件下,XA和UA的峰电流值与浓度在1. 0×10-7～1. 0×10-5mol·L-1范围内成良好线性关系, XA和UA检出限分别为4. 0×10-9mol·L-1和2. 0×10-8mol·L-1.在两种添加水平下,XA和UA加标回收率分别为100. 4% ～111. 4%和96. 3% ～103. 5%,RSD(n＝6)均小于4. 2%和3. 6%.方法用于人体尿液中XA和UA检测,获得满意结果.%The glassy carbon electrode(GCE)was modified by dropping Graphene/Manganese dioxide dispersion,and the 1-octyl-3-methylimidazolium hexafluoro-phosphate(OMIMPF6)was used to prepare the OMIMPF6/Graphene/Manganese dioxide/GCE with the electro-poly-merized method. The modified electrode was analyzed simultaneously for the mixture of xanthine(XA)and uric acid (UA). The result indicated that XA and UA had better electrochemical behavior in the 0. 2 mol·L-1phosphoric acid buffer(pH＝5. 0). Under optimal conditions,the oxidation peak currents of XA and UA were linearly related to the concentration in the range of 1. 0×10-7～1. 0×10-5mol·L-1,and the detection limit of XA and UA was 4. 0×10-9mol·L-1and 2. 0×10-8mol·L-1,respectively. At the two spiked levels,the recoveries of XA and UA were 100. 4% ～111. 4%and 96. 3% ～103. 5%,and the values of RSDs(n＝6)were lower 4. 2%and 3. 6%,respectively. The established method was applied to the determination of the XA and UA in hu-man urine and the satisfactory results were obtained.
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